{"id":54887,"date":"2022-06-27T09:52:06","date_gmt":"2022-06-27T14:52:06","guid":{"rendered":"https:\/\/www.bjultrasonic.com\/how-is-the-microscope-slide-test-done\/"},"modified":"2025-01-21T07:13:13","modified_gmt":"2025-01-21T12:13:13","slug":"how-is-the-microscope-slide-test-done","status":"publish","type":"post","link":"https:\/\/www.bjultrasonic.com\/vi\/how-is-the-microscope-slide-test-done\/","title":{"rendered":"Quy tr\u00ecnh th\u1ef1c hi\u1ec7n x\u00e9t nghi\u1ec7m lam k\u00ednh"},"content":{"rendered":"

Vi\u1ec7c ki\u1ec3m tra m\u1eabu v\u1eadt d\u01b0\u1edbi k\u00ednh hi\u1ec3n vi l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt c\u01a1 b\u1ea3n trong nhi\u1ec1u l\u0129nh v\u1ef1c, t\u1eeb sinh h\u1ecdc, y h\u1ecdc \u0111\u1ebfn khoa h\u1ecdc v\u1eadt li\u1ec7u. Qu\u00e1 tr\u00ecnh n\u00e0y, tuy c\u00f3 v\u1ebb \u0111\u01a1n gi\u1ea3n, nh\u01b0ng l\u1ea1i \u0111\u00f2i h\u1ecfi s\u1ef1 t\u1ec9 m\u1ec9 v\u00e0 ch\u00ednh x\u00e1c \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o k\u1ebft qu\u1ea3 quan s\u00e1t \u0111\u00e1ng tin c\u1eady. B\u00e0i vi\u1ebft n\u00e0y s\u1ebd h\u01b0\u1edbng d\u1eabn chi ti\u1ebft c\u00e1ch th\u1ef1c hi\u1ec7n ki\u1ec3m tra m\u1eabu v\u1eadt tr\u00ean lam k\u00ednh hi\u1ec3n vi.<\/p>\n

Chu\u1ea9n b\u1ecb m\u1eabu v\u1eadt<\/h3>\n

B\u01b0\u1edbc \u0111\u1ea7u ti\u00ean v\u00e0 quan tr\u1ecdng nh\u1ea5t l\u00e0 chu\u1ea9n b\u1ecb m\u1eabu v\u1eadt. T\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i m\u1eabu, ph\u01b0\u01a1ng ph\u00e1p chu\u1ea9n b\u1ecb s\u1ebd kh\u00e1c nhau. M\u1eabu v\u1eadt c\u00f3 th\u1ec3 l\u00e0 t\u1ebf b\u00e0o s\u1ed1ng, m\u00f4, vi sinh v\u1eadt, ho\u1eb7c v\u1eadt li\u1ec7u kh\u00f4ng s\u1ed1ng. M\u1ed9t s\u1ed1 k\u1ef9 thu\u1eadt chu\u1ea9n b\u1ecb m\u1eabu ph\u1ed5 bi\u1ebfn bao g\u1ed3m c\u1eaft l\u00e1t m\u1ecfng, nhu\u1ed9m m\u00e0u, c\u1ed1 \u0111\u1ecbnh, v\u00e0 nh\u00fang paraffin. Vi\u1ec7c chu\u1ea9n b\u1ecb m\u1eabu \u0111\u00fang c\u00e1ch s\u1ebd gi\u00fap quan s\u00e1t r\u00f5 r\u00e0ng c\u00e1c c\u1ea5u tr\u00fac v\u00e0 \u0111\u1eb7c \u0111i\u1ec3m c\u1ee7a m\u1eabu v\u1eadt.<\/p>\n

Chu\u1ea9n b\u1ecb lam k\u00ednh<\/h3>\n

Lam k\u00ednh c\u1ea7n \u0111\u01b0\u1ee3c l\u00e0m s\u1ea1ch k\u1ef9 l\u01b0\u1ee1ng \u0111\u1ec3 tr\u00e1nh b\u1ee5i b\u1ea9n v\u00e0 c\u00e1c t\u1ea1p ch\u1ea5t kh\u00e1c \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn qu\u00e1 tr\u00ecnh quan s\u00e1t. C\u00f3 th\u1ec3 s\u1eed d\u1ee5ng c\u1ed3n ho\u1eb7c c\u00e1c dung d\u1ecbch l\u00e0m s\u1ea1ch chuy\u00ean d\u1ee5ng \u0111\u1ec3 lau s\u1ea1ch lam k\u00ednh. Sau khi lau, c\u1ea7n \u0111\u1ec3 lam k\u00ednh kh\u00f4 ho\u00e0n to\u00e0n tr\u01b0\u1edbc khi \u0111\u1eb7t m\u1eabu v\u1eadt l\u00ean.<\/p>\n

\u0110\u1eb7t m\u1eabu v\u1eadt l\u00ean lam k\u00ednh<\/h3>\n

M\u1eabu v\u1eadt \u0111\u01b0\u1ee3c \u0111\u1eb7t l\u00ean lam k\u00ednh m\u1ed9t c\u00e1ch c\u1ea9n th\u1eadn, tr\u00e1nh l\u00e0m v\u1ee1 ho\u1eb7c bi\u1ebfn d\u1ea1ng m\u1eabu. \u0110\u1ed1i v\u1edbi m\u1eabu v\u1eadt l\u1ecfng, c\u00f3 th\u1ec3 d\u00f9ng pipet nh\u1ecf gi\u1ecdt m\u1ed9t l\u01b0\u1ee3ng v\u1eeba \u0111\u1ee7 l\u00ean lam k\u00ednh. \u0110\u1ed1i v\u1edbi m\u1eabu v\u1eadt r\u1eafn, c\u1ea7n s\u1eed d\u1ee5ng k\u1eb9p ho\u1eb7c d\u1ee5ng c\u1ee5 chuy\u00ean d\u1ee5ng \u0111\u1ec3 \u0111\u1eb7t m\u1eabu v\u1eadt l\u00ean lam k\u00ednh.<\/p>\n

\u0110\u1eady lamen<\/h3>\n

Lamen l\u00e0 m\u1ed9t mi\u1ebfng k\u00ednh m\u1ecfng, vu\u00f4ng ho\u1eb7c h\u00ecnh ch\u1eef nh\u1eadt, \u0111\u01b0\u1ee3c \u0111\u1eb7t l\u00ean tr\u00ean m\u1eabu v\u1eadt \u0111\u1ec3 b\u1ea3o v\u1ec7 m\u1eabu v\u00e0 t\u1ea1o ra m\u1ed9t b\u1ec1 m\u1eb7t ph\u1eb3ng, \u0111\u1ed3ng nh\u1ea5t cho vi\u1ec7c quan s\u00e1t. Khi \u0111\u1eady lamen, c\u1ea7n \u0111\u1eb7t nh\u1eb9 nh\u00e0ng v\u00e0 tr\u00e1nh t\u1ea1o b\u1ecdt kh\u00ed.<\/p>\n

Quan s\u00e1t d\u01b0\u1edbi k\u00ednh hi\u1ec3n vi<\/h3>\n

Sau khi \u0111\u00e3 chu\u1ea9n b\u1ecb xong lam k\u00ednh, \u0111\u1eb7t lam k\u00ednh l\u00ean b\u00e0n \u0111\u1eb7t m\u1eabu c\u1ee7a k\u00ednh hi\u1ec3n vi. B\u1eaft \u0111\u1ea7u quan s\u00e1t v\u1edbi v\u1eadt k\u00ednh c\u00f3 \u0111\u1ed9 ph\u00f3ng \u0111\u1ea1i th\u1ea5p nh\u1ea5t, sau \u0111\u00f3 t\u0103ng d\u1ea7n \u0111\u1ed9 ph\u00f3ng \u0111\u1ea1i \u0111\u1ec3 quan s\u00e1t chi ti\u1ebft h\u01a1n. \u0110i\u1ec1u ch\u1ec9nh ti\u00eau c\u1ef1 v\u00e0 \u00e1nh s\u00e1ng \u0111\u1ec3 c\u00f3 \u0111\u01b0\u1ee3c h\u00ecnh \u1ea3nh r\u00f5 n\u00e9t nh\u1ea5t.<\/p>\n

M\u1ed9t s\u1ed1 l\u01b0u \u00fd quan tr\u1ecdng<\/h3>\n

Vi\u1ec7c l\u01b0u tr\u1eef lam k\u00ednh sau khi quan s\u00e1t c\u0169ng r\u1ea5t quan tr\u1ecdng. Lam k\u00ednh c\u1ea7n \u0111\u01b0\u1ee3c l\u00e0m s\u1ea1ch v\u00e0 b\u1ea3o qu\u1ea3n \u0111\u00fang c\u00e1ch \u0111\u1ec3 c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng l\u1ea1i cho c\u00e1c l\u1ea7n quan s\u00e1t sau.<\/p>\n\n\n\n\n\n\n\n\n
K\u1ef9 thu\u1eadt chu\u1ea9n b\u1ecb m\u1eabu<\/th>\nM\u00f4 t\u1ea3<\/th>\n\u1ee8ng d\u1ee5ng<\/th>\n<\/tr>\n<\/thead>\n
C\u1eaft l\u00e1t m\u1ecfng<\/td>\nC\u1eaft m\u1eabu v\u1eadt th\u00e0nh l\u00e1t m\u1ecfng b\u1eb1ng microtome<\/td>\nQuan s\u00e1t c\u1ea5u tr\u00fac b\u00ean trong c\u1ee7a m\u00f4<\/td>\n<\/tr>\n
Nhu\u1ed9m m\u00e0u<\/td>\nS\u1eed d\u1ee5ng thu\u1ed1c nhu\u1ed9m \u0111\u1ec3 l\u00e0m n\u1ed5i b\u1eadt c\u00e1c c\u1ea5u tr\u00fac t\u1ebf b\u00e0o<\/td>\nPh\u00e2n bi\u1ec7t c\u00e1c lo\u1ea1i t\u1ebf b\u00e0o v\u00e0 c\u1ea5u tr\u00fac<\/td>\n<\/tr>\n
C\u1ed1 \u0111\u1ecbnh<\/td>\nS\u1eed d\u1ee5ng h\u00f3a ch\u1ea5t \u0111\u1ec3 gi\u1eef nguy\u00ean c\u1ea5u tr\u00fac t\u1ebf b\u00e0o<\/td>\nB\u1ea3o qu\u1ea3n m\u1eabu v\u1eadt l\u00e2u d\u00e0i<\/td>\n<\/tr>\n
Nh\u00fang paraffin<\/td>\nNh\u00fang m\u1eabu v\u1eadt v\u00e0o paraffin \u0111\u1ec3 t\u1ea1o kh\u1ed1i c\u1ee9ng<\/td>\nC\u1eaft l\u00e1t m\u1ecfng d\u1ec5 d\u00e0ng h\u01a1n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Vi\u1ec7c th\u1ef1c hi\u1ec7n \u0111\u00fang c\u00e1c b\u01b0\u1edbc trong quy tr\u00ecnh ki\u1ec3m tra m\u1eabu v\u1eadt tr\u00ean lam k\u00ednh hi\u1ec3n vi s\u1ebd \u0111\u1ea3m b\u1ea3o k\u1ebft qu\u1ea3 quan s\u00e1t ch\u00ednh x\u00e1c v\u00e0 \u0111\u00e1ng tin c\u1eady, \u0111\u00f3ng g\u00f3p quan tr\u1ecdng v\u00e0o vi\u1ec7c nghi\u00ean c\u1ee9u v\u00e0 ph\u00e2n t\u00edch trong nhi\u1ec1u l\u0129nh v\u1ef1c khoa h\u1ecdc.<\/p>\n","protected":false},"excerpt":{"rendered":"

Vi\u1ec7c ki\u1ec3m tra m\u1eabu v\u1eadt d\u01b0\u1edbi k\u00ednh hi\u1ec3n vi l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt c\u01a1 b\u1ea3n trong nhi\u1ec1u l\u0129nh v\u1ef1c, t\u1eeb sinh h\u1ecdc, y h\u1ecdc \u0111\u1ebfn khoa h\u1ecdc v\u1eadt li\u1ec7u. Qu\u00e1 tr\u00ecnh n\u00e0y, tuy c\u00f3 v\u1ebb \u0111\u01a1n gi\u1ea3n, nh\u01b0ng l\u1ea1i \u0111\u00f2i h\u1ecfi s\u1ef1 t\u1ec9 m\u1ec9 v\u00e0 ch\u00ednh x\u00e1c \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o k\u1ebft qu\u1ea3 quan s\u00e1t \u0111\u00e1ng<\/p>\n","protected":false},"author":1,"featured_media":20118,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6406],"tags":[],"class_list":["post-54887","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","prodpage-classic"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/posts\/54887","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/comments?post=54887"}],"version-history":[{"count":0,"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/posts\/54887\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/media\/20118"}],"wp:attachment":[{"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/media?parent=54887"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/categories?post=54887"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bjultrasonic.com\/vi\/wp-json\/wp\/v2\/tags?post=54887"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}